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  Endosome fusion and microtubule-based dynamics in the early endocytic pathway of Dictyostelium

Clarke, M., Köhler, J., Heuser, J., & Gerisch, G. (2002). Endosome fusion and microtubule-based dynamics in the early endocytic pathway of Dictyostelium. Traffic, 3(11), 791-800.

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Genre: Journal Article
Alternative Title : Traffic

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 Creators:
Clarke, M., Author
Köhler, J.1, Author              
Heuser, J., Author
Gerisch, G.1, Author              
Affiliations:
1Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              

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Free keywords: Dictyostelium; endosome; macropinocytosis; microtubule; vesicle traffic
 Abstract: Dictyostelium amoebae, like mammalian macrophages, take up fluid by macropinocytosis. The present study used fluorescent fluid-phase markers and GFP-labeled microtubules to visualize the uptake, dynamics, and fusion of early endosomes in Dictyostelium. Consecutive labeling with two fluorescent fluid- phase markers demonstrated that within the first few minutes after uptake, new macropinosomes underwent fusion with pre- existing endosomes. The fusing endosomes, which represent the mixing compartment, displayed extreme shape changes and rapid transport about the cell in association with microtubules. The great plasticity of endosomes at this stage of maturation was also evident by electron microscopy. The constant undulatory motion of microtubules was implemental in establishing contact with endosomes. Treatment of cells with agents that selectively disrupted either actin filaments or microtubules confirmed that endosome dynamics were microtubule based. Further maturation of endosomes led to loss of pleiomorphy in favor of a spherical shape, inability to fuse with new macropinosomes, and diminished motility.

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Language(s): eng - English
 Dates: 2002-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41346
ISI: 000178617000004
 Degree: -

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Title: Traffic
  Alternative Title : Traffic
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 3 (11) Sequence Number: - Start / End Page: 791 - 800 Identifier: ISSN: 1398-9219