English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells

Gruss, O. J., Wittmann, M., Yokoyama, H., Pepperkok, R., Kufer, T., Sillje, H., et al. (2002). Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Nature Cell Biology, 4(11), 871-879.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : Nat. Cell Biol.

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Gruss, O. J., Author
Wittmann, M., Author
Yokoyama, H., Author
Pepperkok, R., Author
Kufer, T.1, Author              
Sillje, H.1, Author              
Karsenti, E., Author
Mattaj, I. W., Author
Vernos, I., Author
Affiliations:
1Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

Content

show
hide
Free keywords: -
 Abstract: In Xenopus laevis egg extracts, TPX2 is required for the Ran- GTP-dependent assembly of microtubules around chromosomes. Here we show that interfering with the function of the human homologue of TPX2 in HeLa cells causes defects in microtubule organization during mitosis. Suppressing the expression of human TPX2 by RNA interference leads to the formation of two microtubule asters that do not interact and do not form a spindle. Our results suggest that in vivo, even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells.

Details

show
hide
Language(s): eng - English
 Dates: 2002-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41349
ISI: 000179137700016
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Cell Biology
  Alternative Title : Nat. Cell Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 4 (11) Sequence Number: - Start / End Page: 871 - 879 Identifier: ISSN: 1465-7392