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  The integration of a major hepatitis B virus gene into cell- cycle synchronized carrot cell suspension cultures and its expression in regenerated carrot plants

Imani, J., Berting, A., Nitsche, S., Schaefer, S., Gerlich, W. H., & Neumann, K. H. (2002). The integration of a major hepatitis B virus gene into cell- cycle synchronized carrot cell suspension cultures and its expression in regenerated carrot plants. Plant Cell Tissue and Organ Culture, 71(2), 157-164.

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Genre: Journal Article
Alternative Title : Plant Cell Tissue Organ Cult.

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 Creators:
Imani, J., Author
Berting, A.1, Author           
Nitsche, S., Author
Schaefer, S., Author
Gerlich, W. H., Author
Neumann, K. H., Author
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1External Organizations, ou_persistent22              

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Free keywords: cell cycle synchronization; Daucus; FDU/Thymidine; hepatitis B; transformation efficiency; transgenic strains
 Abstract: The percentage of cells successfully transformed by Agrobacteria is usually very low (not more than 10% and usually much less; Thomas et al., 1989). In this report we show that in carrot (Daucus carota L., ssp. sativus) cell suspensions transformation efficiency was strongly improved by using cell cycle synchronized cells. Fluorodesoxyuridine (FDU) was added for 24 h to inhibit thymidine synthesis. This blocked the cell cycle at the transition from G1- to S-phase. Then the block was released by applying thymidine. A high rate of transformation was obtained when Agrobacterium tumefaciens was added concurrently with thymidine. As examples of efficient and long term foreign gene expression in transgenic cells, the reporter enzyme beta-glucuronidase (GUS) as a model as well as the major hepatitis B virus surface protein were used. Both genes were linked to the MAS promoter. In carrot cell suspensions containing the viral gene, the corresponding viral protein was produced. In roots of mature transgenic carrot plants generated through somatic embryogenesis and raised in soil as well as in callus cultures derived thereof, the viral protein was also produced.

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Language(s): eng - English
 Dates: 2002-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 49910
ISI: 000177678800009
 Degree: -

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Title: Plant Cell Tissue and Organ Culture
  Alternative Title : Plant Cell Tissue Organ Cult.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 71 (2) Sequence Number: - Start / End Page: 157 - 164 Identifier: ISSN: 0167-6857