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  Identification of macromolecular complexes in cryoelectron tomograms of phantom cells

Frangakis, A. S., Böhm, J., Förster, F., Nickell, S., Nicastro, D., Typke, D., et al. (2002). Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. Proceedings of the National Academy of Sciences of the United States of America, 99(22), 14153-14158.

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Genre: Journal Article
Alternative Title : Proc. Natl. Acad. Sci. U. S. A.

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 Creators:
Frangakis, A. S.1, Author              
Böhm, J.2, Author              
Förster, F.2, 3, Author              
Nickell, S.2, Author              
Nicastro, D.1, Author              
Typke, D.2, Author              
Hegerl, R.2, Author              
Baumeister, W.2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
3Förster, Friedrich / Modeling of Protein Complexes, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565148              

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 Abstract: Electron tomograms of intact frozen-hydrated cells are essentially three-dimensional images of the entire proteome of the cell, and they depict the whole network of macromolecular interactions. However, this information is not easily accessible because of the poor signal-to-noise ratio of the tomograms and the crowded nature of the cytoplasm. Here, we describe a template matching algorithm that is capable of detecting and identifying macromolecules in tomographic volumes in a fully automated manner. The algorithm is based on nonlinear cross correlation and incorporates elements of multivariate statistical analysis. Phantom cells, i.e., lipid vesicles filled with macromolecules, provide a realistic experimental scenario for an assessment of the fidelity of this approach. At the current resolution of approximate to4 nm, macromolecules in the size range of 0.5-1 MDa can be identified with good fidelity.

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Language(s): eng - English
 Dates: 2002-10-29
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41547
ISI: 000178967400036
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Alternative Title : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 99 (22) Sequence Number: - Start / End Page: 14153 - 14158 Identifier: ISSN: 0027-8424