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  Gene structure and functional analysis of the mouse nidogen-2 gene: Nidogen-2 is not essential for basement membrane formation in mice

Schymeinsky, J., Nedbal, S., Miosge, N., Poschl, E., Rao, C., Beier, D. R., et al. (2002). Gene structure and functional analysis of the mouse nidogen-2 gene: Nidogen-2 is not essential for basement membrane formation in mice. Molecular and Cellular Biology, 22(19), 6820-6830.

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Genre: Journal Article
Alternative Title : Mol. Cell. Biol.

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 Creators:
Schymeinsky, J.1, Author              
Nedbal, S.1, Author              
Miosge, N., Author
Poschl, E., Author
Rao, C., Author
Beier, D. R., Author
Skarnes, W. C., Author
Timpl, R.2, Author              
Bader, B. L.2, Author              
Affiliations:
1Ullrich, Axel / Molecular Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565172              
2Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

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 Abstract: Nidogens are highly conserved proteins in vertebrates and invertebrates and are found in almost all basement membranes. According to the classical hypothesis of basement membrane organization, nidogens connect the laminin and collagen IV networks, so stabilizing the basement membrane, and integrate other proteins. In mammals two nidogen proteins, nidogen-1 and nidogen-2, have been discovered. Nidogen-2 is typically enriched in endothelial basement membranes, whereas nidogen-1 shows broader localization in most basement membranes. Surprisingly, analysis of nidogen-1 gene knockout mice presented evidence that nidogen-1 is not essential for basement membrane formation and may be compensated for by nidogen-2. In order to assess the structure and in vivo function of the nidogen-2 gene in mice, we cloned the gene and determined its structure and chromosomal location. Next we analyzed mice carrying an insertional mutation in the nidogen-2 gene that was generated by the secretory gene trap approach. Our molecular and biochemical characterization identified the mutation as a phenotypic null allele. Nidogen-2-deficient mice show no overt abnormalities and are fertile, and basement membranes appear normal by ultrastructural analysis and immunostaining. Nidogen- 2 deficiency does not lead to hemorrhages in mice as one may have expected. Our results show that nidogen-2 is not essential for basement membrane formation or maintenance.

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Language(s): eng - English
 Dates: 2002-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41646
ISI: 000177961900017
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Title: Molecular and Cellular Biology
  Alternative Title : Mol. Cell. Biol.
Source Genre: Journal
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Pages: - Volume / Issue: 22 (19) Sequence Number: - Start / End Page: 6820 - 6830 Identifier: ISSN: 0270-7306