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  A talin fragment as an actin trap visualizing actin flow in chemotaxis, endocytosis, and cytokinesis

Weber, I., Niewöhner, J., Du, A. P., Röhrig, U., & Gerisch, G. (2002). A talin fragment as an actin trap visualizing actin flow in chemotaxis, endocytosis, and cytokinesis. Cell Motility and the Cytoskeleton, 53(2), 136-149.

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Genre: Journal Article
Alternative Title : Cell Motil. Cytoskeleton

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 Creators:
Weber, I.1, Author              
Niewöhner, J.1, Author              
Du, A. P.1, Author              
Röhrig, U.1, Author              
Gerisch, G.1, Author              
Affiliations:
1Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              

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Free keywords: actin flow; cleavage furrow; Dictyostelium; green fluorescent protein; mitosis
 Abstract: A C-terminal 63-kDa fragment of talin A from Dictyostelium discoideum forms a slowly dissociating complex with F-actin in vitro. This talin fragment (TalC63) has been tagged with GFP and used as a trap for actin filaments in chemotactic cell movement, endocytosis, and mitotic cell division. TalC63 efficiently sequesters actin filaments in vivo. Its translocation reflects the direction and efficiency of an actin flow. Along the body of a migrating Dictyostelium cell, this flow is directed from the front to the tail. If during chemotaxis one or two new fronts are induced, the flow is always directed away from these fronts. The flow thus reflects the re-programming of cell polarity in response to changing gradients of chemoattractant. In endocytosis, the fluorescent complexes are translocated to the base of a phagocytic or macropinocytic cup. During mitosis, the complexes of F-actin with TalC63 accumulate within the midzone of anaphase cells. If TalC63 is strongly expressed, the entire cleavage furrow is filled out by sequestered actin filaments, and cytokinesis is severely impaired. These cells are considered to mimic the phenotype of mutants deficient in the shredding of actin filaments that normally occurs in the mid-zone of a dividing cell. Cell Motil. Cytoskeleton 53: 136-149, 2002. (C) 2002 Wiley-Liss. Inc.

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Language(s): eng - English
 Dates: 2002-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41728
ISI: 000178272000005
 Degree: -

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Title: Cell Motility and the Cytoskeleton
  Alternative Title : Cell Motil. Cytoskeleton
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 53 (2) Sequence Number: - Start / End Page: 136 - 149 Identifier: ISSN: 0886-1544