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Free keywords:
binding affinity; cell adhesion; collagen; integrins; structure elucidation
Abstract:
Checking the register: Cell-adhesion processes are mediated by conformation-dependent binding of collagen type IV to integrins, Two heterotrimeric collagen peptides that contain the residue-457-468 sequence of the cell-adhesion epitope of the alpha1 and alpha2 chains in collagen type IV were synthesized and the three chains were assembled into the alpha1alpha2alpha1' and alpha2alpha1alpha1' registers (see figure). Binding and modeling experiments allowed identification of the latter register as the most plausible alignment of the chains in natural collagen type IV.
