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  Slow exchange in the chromophore of a green fluorescent protein variant

Seifert, M. H., Ksiazek, D., Azim, M. K., Smialowski, P., Budisa, N., & Holak, T. A. (2002). Slow exchange in the chromophore of a green fluorescent protein variant. Journal of the American Chemical Society, 124(27), 7932-7942.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-6ECE-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-6ECF-2
Genre: Journal Article
Alternative Title : J. Am. Chem. Soc.

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Seifert, M. H., Author
Ksiazek, D., Author
Azim, M. K.1, Author              
Smialowski, P.2, 3, Author              
Budisa, N.4, Author              
Holak, T. A.5, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Huber, Robert / Structure Research, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565155              
3Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
4Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              
5Holak, Tad / NMR Spectroscopy, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565154              

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 Abstract: Green fluorescent protein and its mutants have become valuable tools in molecular biology. They also provide systems rich in photophysical and photochemical phenomena of which an understanding is important for the development of new and optimized variants of GFP. Surprisingly, not a single NMR study has been reported on GFPs until now, possibly because of their high tendency to aggregate. Here, we report the F-19 nuclear magnetic resonance (NMR) studies on mutants of the green fluorescent protein (GFP) and cyan fluorescent protein (CFP) labeled with fluorinated tryptophans that enabled the detection of slow molecular motions in these proteins. The concerted use of dynamic NMR and F-19 relaxation measurements, supported by temperature, concentration- and folding-dependent experiments provides direct evidence for the existence of a slow exchange process between two different conformational states of CFP. F- 19 NMR relaxation and line shape analysis indicate that the time scale of exchange between these states is in the range of 1.2-1.4 ms. Thermodynamic analysis revealed a difference in enthalpy DeltaH(0) = (18.2 +/- 3.8) kJ/mol and entropy TDeltaS(0) = (19.6 +/- 1.2) kJ/mol at T = 303 K for the two states involved in the exchange process, indicating an entropy- enthalpy compensation. The free energy of activation was estimated to be approximately 60 kJ/mol. Exchange between two conformations, either of the chromophore itself or more likely of the closely related histidine 148, is suggested to be the structural process underlying the conformational mobility of GFPs. The possibility to generate a series of single-atom exchanges ("atomic mutations") like H --> F in this study offers a useful approach for characterizing and quantifying dynamic processes in proteins by NMR.

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Language(s): eng - English
 Dates: 2002-07-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 31392
ISI: 000176612400024
 Degree: -

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Title: Journal of the American Chemical Society
  Alternative Title : J. Am. Chem. Soc.
Source Genre: Journal
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Pages: - Volume / Issue: 124 (27) Sequence Number: - Start / End Page: 7932 - 7942 Identifier: ISSN: 0002-7863