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  Structural properties of a collagenous heterotrimer that mimics the collagenase cleavage site of collagen type I

Fiori, S., Saccà, B., & Moroder, L. (2002). Structural properties of a collagenous heterotrimer that mimics the collagenase cleavage site of collagen type I. Journal of Molecular Biology, 319(5), 1235-1242.

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Genre: Journal Article
Alternative Title : J. Mol. Biol.

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 Creators:
Fiori, S.1, Author              
Saccà, B.1, Author              
Moroder, L.1, Author              
Affiliations:
1Moroder, Luis / Bioorganic Chemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565160              

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Free keywords: collagenase substrate; collagenous heterotrimers; conformation; NMR; proton exchange
 Abstract: Collagens contain sequence- and conformation-dependent epitopes responsible for their digestion by collagenases at specific loci. A synthetic heterotrimer construct containing the collagenase cleavage site of collagen type I was found to mimic perfectly native collagen in terms of selectivity and mode of enzymatic degradation. The NMR conformational analysis of this molecule clearly revealed the presence of two structural domains, i.e. a triple helix spanning the Gly-Pro-Hyp repeats and a less ordered portion corresponding to the collagenase cleavage site where the three chains are aligned in extended conformation with loose interchain contacts. These structural properties allow for additional insights into the very particular mechanism of collagen digestion by collagenases. (C) 2002 Elsevier Science Ltd. All rights reserved.

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Language(s): eng - English
 Dates: 2002-06-21
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 31396
ISI: 000176687400019
 Degree: -

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Title: Journal of Molecular Biology
  Alternative Title : J. Mol. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 319 (5) Sequence Number: - Start / End Page: 1235 - 1242 Identifier: ISSN: 0022-2836