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  The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase

Kirsh, O., Seeler, J. S., Pichler, A., Gast, A., Muller, S., Miska, E., et al. (2002). The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase. EMBO Journal, 21(11), 2682-2691.

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Genre: Journal Article
Alternative Title : Embo J.

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 Creators:
Kirsh, O., Author
Seeler, J. S., Author
Pichler, A.1, Author              
Gast, A., Author
Muller, S.2, Author
Miska, E., Author
Mathieu, M., Author
Harel-Bellan, A., Author
Kouzarides, T., Author
Melchior, F.1, Author              
Dejean, A., Author
Affiliations:
1Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              
2External Organizations, ou_persistent22              

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Free keywords: E3 ligase; HDACs; nuclear pore complex; SUMO; ubiquitin-like modification
 Abstract: Transcriptional repression mediated through histone deacetylation is a critical component of eukaryotic gene regulation. Here we demonstrate that the class II histone deacetylase HDAC4 is covalently modified by the ubiquitin- related SUMO-1 modifier. A sumoylation-deficient point mutant (HDAC4-K559R) shows a slightly impaired ability to repress transcription as well as reduced histone deacetylase activity. The ability of HDAC4 to self-aggregate is a prerequisite for proper sumoylation in vivo. Calcium/calmodulin-dependent protein kinase (CaMK) signalling, which induces nuclear export, abrogates SUMO-1 modification of HDAC4. Moreover, the modification depends on the presence of an intact nuclear localization signal and is catalysed by the nuclear pore complex (NPC) RanBP2 protein, a factor newly identified as a SUMO E3 ligase. These findings suggest that sumoylation of HDAC4 takes place at the NPC and is coupled to its nuclear import. Finally, modification experiments indicate that the MEF2-interacting transcription repressor (MITR) as well as HDAC1 and -6 are similarly SUMO modified, indicating that sumoylation may be an important regulatory mechanism for the control of transcriptional repression mediated by both class I and II HDACs.

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Language(s): eng - English
 Dates: 2002-06-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 29128
ISI: 000175912500018
 Degree: -

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Title: EMBO Journal
  Alternative Title : Embo J.
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 21 (11) Sequence Number: - Start / End Page: 2682 - 2691 Identifier: ISSN: 0261-4189