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  Specific ablation of the nidogen-binding site in the laminin gamma 1 chain interferes with kidney and lung development

Willem, M., Miosge, N., Halfter, W., Smyth, N., Jannetti, I., Burghart, E., et al. (2002). Specific ablation of the nidogen-binding site in the laminin gamma 1 chain interferes with kidney and lung development. Development, 129(11), 2711-2722.

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Genre: Journal Article
Alternative Title : Development

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 Creators:
Willem, M.1, Author              
Miosge, N., Author
Halfter, W., Author
Smyth, N., Author
Jannetti, I., Author
Burghart, E., Author
Timpl, R.2, Author              
Mayer, U.2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

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Free keywords: basement membrane; Wolffian duct; kidney; lung; morphogenesis; mouse
 Abstract: Basement membrane assembly is of crucial importance in the development and function of tissues and during embryogenesis. Nidogen 1 was thought to be central in the assembly processes, connecting the networks formed by collagen type IV and laminins, however, targeted inactivation of nidogen I resulted in no obvious phenotype. We have now selectively deleted the sequence coding for the 56 amino acid nidogen-binding site, gamma1III4, within the Lamc1 gene by gene targeting. Here, we show that mice homozygous for the deletion die immediately after birth, showing renal agenesis and impaired lung development. These developmental defects were attributed to locally restricted ruptures in the basement membrane of the elongating Wolffian duct and of alveolar sacculi. These data demonstrate that an interaction between two basement membrane proteins is required for early kidney morphogenesis in vivo.

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Language(s): eng - English
 Dates: 2002-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 31372
ISI: 000176471600014
 Degree: -

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Title: Development
  Alternative Title : Development
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 129 (11) Sequence Number: - Start / End Page: 2711 - 2722 Identifier: ISSN: 0950-1991