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  Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication

Stucke, V. M., Sillje, H. H. W., Arnaud, L., & Nigg, E. A. (2002). Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication. EMBO Journal, 21(7), 1723-1732.

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Genre: Journal Article
Alternative Title : Embo J.

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 Creators:
Stucke, V. M.1, Author              
Sillje, H. H. W.1, Author
Arnaud, L., Author
Nigg, E. A.2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

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Free keywords: cell cycle; centrosome duplication; hMps1; mitosis; mitotic spindle checkpoint
 Abstract: Budding yeast Mps1p kinase has been implicated in both the duplication of microtubule-organizing centers and the spindle assembly checkpoint. Here we show that hMps1, the human homolog of yeast Mps1p, is a cell cycle-regulated kinase with maximal activity during M phase. hMps1 localizes to kinetochores and its activity and phosphorylation state increase upon activation of the mitotic checkpoint. By antibody microinjection and siRNA, we demonstrate that hMps1 is required for human cells to undergo checkpoint arrest in response to microtubule depolymerization. In contrast, centrosome (re-)duplication as well as cell division occur in the absence of hMps1. We conclude that hMps1 is required for the spindle assembly checkpoint but not for centrosome duplication.

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Language(s): eng - English
 Dates: 2002-04-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 39309
ISI: 000174992000022
 Degree: -

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Title: EMBO Journal
  Alternative Title : Embo J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 21 (7) Sequence Number: - Start / End Page: 1723 - 1732 Identifier: ISSN: 0261-4189