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  Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli

Lüttgen, H., Robelek, R., Mühlberger, R., Diercks, T., Schuster, S. C., Kohler, P., et al. (2002). Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli. Journal of Molecular Biology, 316(4), 875-885.

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Genre: Journal Article
Alternative Title : J. Mol. Biol.

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 Creators:
Lüttgen, H., Author
Robelek, R., Author
Mühlberger, R., Author
Diercks, T., Author
Schuster, S. C.1, Author              
Kohler, P., Author
Kessler, H., Author
Bacher, A., Author
Richter, G., Author
Affiliations:
1Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              

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Free keywords: antitermination; ribosomal RNA; NMR spectroscopy; surface plasmon spectroscopy; protein/RNA interaction
 Abstract: A recombinant heterodimeric NusB/NusE protein complex of Escherichia coli was expressed under the control of a synthetic mini operon. Surface plasmon resonance measurements showed that the heterodimer complex has substantially higher affinity for the boxA RNA sequence motif of the ribosomal RNA (rrn) operons of E. coli as compared to monomeric NusB protein. Single base exchanges in boxA RNA reduced the affinity of the protein complex up to 15-fold. The impact of base exchanges in the boxA RNA on the interaction with NusB protein was studied by H-1,N- 15 hetero-correlation NMR spectroscopy. Spectra obtained with modified RNA sequences were analysed by a novel generic algorithm. Replacement of bases in the terminal segments of the boxA RNA motif caused minor chemical shift changes as compared to base exchanges in the central part of the dodecameric boxA motif. (C) 2002 Elsevier Science Ltd.

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Language(s): eng - English
 Dates: 2002-03-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 39190
ISI: 000174503400002
 Degree: -

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Title: Journal of Molecular Biology
  Alternative Title : J. Mol. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 316 (4) Sequence Number: - Start / End Page: 875 - 885 Identifier: ISSN: 0022-2836