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  Robotic high-throughput assay for isolating apoptosis-inducing genes

Grimm, S., & Kachel, V. (2002). Robotic high-throughput assay for isolating apoptosis-inducing genes. BioTechniques, 32(3), 670-677.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-6F9C-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-6F9D-8
Genre: Journal Article
Alternative Title : Biotechniques

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 Creators:
Grimm, S.1, Author              
Kachel, V.1, Author              
Affiliations:
1Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

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 Abstract: The need to assign functions to genes will increase in upcoming years because of the accumulation of sequence information from various organisms. Here we present a genetic screen for the systematic functional analysis of genes in mammalian cells. The screen is based on high-throughput, transient transfections of individual cDNAs by a robotic system. This construction uses a moveable pipetting head with a novel 96-channel parallel tube- pump array and an integrated de-lidding device. Because of the robot design, the processing speed can reach 2000 transfections/h. We demonstrate the suitability of the screen to isolate apoptosis-inducing genes as a first read-out. This is achieved in combination with co-transfected reporter plasmids to produce a novel and convenient assay for apoptosis induction.

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Language(s): eng - English
 Dates: 2002-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 39146
ISI: 000174331000024
 Degree: -

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Title: BioTechniques
  Alternative Title : Biotechniques
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 32 (3) Sequence Number: - Start / End Page: 670 - 677 Identifier: ISSN: 0736-6205