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  Recombinant Kv1.3 potassium channels stabilize tonic firing of cultured rat hippocampal neurons

Kupper, J., Prinz, A. A., & Fromherz, P. (2002). Recombinant Kv1.3 potassium channels stabilize tonic firing of cultured rat hippocampal neurons. Pflugers Archiv-European Journal of Physiology, 443(4), 541-547.

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Genre: Journal Article
Alternative Title : Pflugers Arch.

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 Creators:
Kupper, J.1, Author              
Prinz, A. A.1, Author              
Fromherz, P.1, Author              
Affiliations:
1Fromherz, Peter / Membrane and Neurophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565146              

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Free keywords: K+ channel; action potential; hippocampus; margatoxin; green fluorescent protein
 Abstract: We transfected cultured hippocampal neurons with the cDNA of the voltage-gated K+ channel Kv1.3 to investigate the mechanisms by which a specific ion channel influences excitability. In transfected neurons under voltage clamp we observed an additional outward current that was blocked selectively by margatoxin. Under current-clamp conditions, Kv1.3-expressing neurons fired tonically over a wide range of stimulation intensity. In non-transfected neurons, or in Kv1.3- expressing cells blocked with margatoxin, only a few action potentials were elicited before a stationary depolarized state was reached. We attribute the specific effect of Kv1.3 to its particularly slow deactivation near the resting potential. A computational model showed that a continuous outwards current arises in Kv1.3-expressing neurons during the interspike intervals. It expands the dynamic range so that these neurons still fire tonically at stimulus current intensities at which non-transfected cells have already been driven into a stationary depolarized state.

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Language(s): eng - English
 Dates: 2002-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 39155
ISI: 000174363000005
 Degree: -

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Title: Pflugers Archiv-European Journal of Physiology
  Alternative Title : Pflugers Arch.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 443 (4) Sequence Number: - Start / End Page: 541 - 547 Identifier: ISSN: 0031-6768