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  Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells

Grimm, D., Bauer, J., Kossmehl, P., Shakibaei, M., Schonberger, J., Pickenhahn, H., et al. (2002). Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells. FASEB Journal, 16(2), U55-U81.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-6FDC-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-6FDD-9
Genre: Journal Article
Alternative Title : Faseb J.

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 Creators:
Grimm, D., Author
Bauer, J.1, Author              
Kossmehl, P., Author
Shakibaei, M., Author
Schonberger, J., Author
Pickenhahn, H., Author
Schulze-Tanzil, G., Author
Vetter, R., Author
Eilles, C., Author
Paul, M., Author
Cogoli, A., Author
Affiliations:
1Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: extracellular matrix proteins; hormones; clinorotation; thyroid tumor; programmed cell death
 Abstract: This study focuses on the effects of simulated microgravity (0g) on the human follicular thyroid carcinoma cell line ML-1. Cultured on a three-dimensional clinostat, ML-1 cells formed three-dimensional MCTSs (MCTS diameter: 0.3+/-0.01 mm). After 24 and 48 h of clinorotation, the cells significantly decreased fT3 and fT4 secretion but up-regulated the thyroid-stimulating hormone-receptor expression as well as the production of vimentin, vinculin, and extracellular matrix proteins (collagen I and III, laminin, fibronectin, chondroitin sulfate) compared with controls. Furthermore, ML-1 cells grown on the clinostat showed elevated amounts of the apoptosis-associated Fas protein, of p53, and of bax but showed reduced quantities of bcl-2. In addition, signs of apoptosis became detectable, as assessed by terminal deoxynucleotidyl transferase-mediated dUTP digoxigenin nick end labeling, 4', 6-diamidino-2-phenylindole staining, DNA laddering, and 85-kDa apoptosis-related cleavage fragments. These fragments resulted from enhanced 116-kDa poly(ADP-ribose)polymerase (PARP) activity and apoptosis. These observations suggest that clinorotation elevates intermediate filaments, cell adhesion molecules, and extracellular matrix proteins and simultaneously induces apoptosis in follicular thyroid cancer cells. In conclusion, our experiments could provide a regulatory basis for the finding that astronauts show low thyroid hormone levels after space flight, which may be explained by the increase of apoptosis in thyrocytes as a result of simulated 0g.

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Language(s): eng - English
 Dates: 2002-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 41688
ISI: 000174203700003
 Degree: -

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Title: FASEB Journal
  Alternative Title : Faseb J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 16 (2) Sequence Number: - Start / End Page: U55 - U81 Identifier: ISSN: 0892-6638