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  Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium

Gerisch, G., Heuser, J., & Clarke, M. (2002). Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium. Cell Biology International, 26(10), 845-852.

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Genre: Journal Article
Alternative Title : Cell Biol. Int.

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 Creators:
Gerisch, G.1, Author              
Heuser, J., Author
Clarke, M., Author
Affiliations:
1Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              

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Free keywords: contractile vacuole; Dictyostelium; membrane trafficking; osmoregulation; rab GTPase; vacuolar H+-ATPase
 Abstract: The contractile vacuole complex of Dictyostelium is the paradigm of a membrane system that undergoes tubular-vesicular transitions during its regular cycle of activities. This system acts as an osmoregulatory organelle in freshwater amoebae and protozoa. It collects fluid in a network of tubules and cisternae, and pumps it out of the cell through transient pores in the plasma membrane. Tubules and vacuoles are interconvertible. The tubular channels are associated with the cortical actin network and are capable of moving and fusing. The contractile vacuole complex is separate from vesicles of the endosomal pathway and preserves its identity in a dispersed state during cell division. We outline techniques to visualize the contractile vacuole system by electron and light microscopy. Emphasis is placed on GFP-fusion proteins that allow visualization of the dynamics of the contractile vacuole network in living cells. Proteins that control activities of this specialized organelle in Dictyostelium have been conserved during evolution and also regulate membrane trafficking in man. (C) 2002 Elsevier Science Ltd. All rights reserved.

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Language(s): eng - English
 Dates: 2002
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 41766
ISI: 000179428900002
 Degree: -

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Title: Cell Biology International
  Alternative Title : Cell Biol. Int.
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 26 (10) Sequence Number: - Start / End Page: 845 - 852 Identifier: ISSN: 1065-6995