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Free keywords:
basement membrane; cell-matrix interaction; radioimmunoassay; recombinant protein
Abstract:
The globular domain IVa from the short arm region of mouse laminin alpha5 chain was obtained by recombinant production and shown to be a cell-adhesive substrate and to bind alphaV beta3 integrin in solid-phase assays. These interactions were blocked by RGD peptides and a restricted panel of anti-integrin antibodies. The two RGD sequences present in alpha 5lVa were shown by site-directed mutagenesis to make different contributions to cell adhesion but were equivalent in binding alphaV beta3 integrin. A quantitative radioimmuno-inhibition assay was established based on domain beta 5IVa which demonstrated distinct amounts of a5 chain in various tissues, particularly in vessel walls. There it could play a role in angiogenesis steps requiring RGD-dependent integrins. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.