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  Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion

Frangakis, A. S., & Hegerl, R. (2001). Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion. Journal of Structural Biology, 135(3), 239-250.

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Genre: Journal Article
Alternative Title : J. Struct. Biol

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 Creators:
Frangakis, A. S.1, Author              
Hegerl, R.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Biochemistry & Biophysics in Current Contents(R)/Life Sciences.
 Abstract: Electron tomography is a powerful technique capable of giving unique insights into the three-dimensional structural organization of pleomorphic biological objects. However, visualization and interpretation of the resulting volumetric data are hampered by an extremely low signal-to-noise ratio, especially when ice-embedded biological specimens are investigated. Usually, isosurface representation or volume rendering of such data is hindered without any further signal enhancement. We propose a novel technique for noise reduction based on nonlinear anisotropic diffusion. The approach combines efficient noise reduction with excellent signal preservation and is clearly superior to conventional methods (e.g., low-pass and median filtering) and invariant wavelet transform filtering. The gain in the signal-to-noise ratio is verified and demonstrated by means of Fourier shell correlation. Improved visualization performance after processing the 3D images is demonstrated with two examples, tomographic reconstructions of chromatin and of a mitochondrion. Parameter settings and discretization stencils are presented in detail. (C) 2001 Elsevier Science. [References: 14]

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 Dates: 2001
 Publication Status: Published in print
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 Identifiers: eDoc: 318433
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Title: Journal of Structural Biology
  Alternative Title : J. Struct. Biol
Source Genre: Journal
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Pages: - Volume / Issue: 135 (3) Sequence Number: - Start / End Page: 239 - 250 Identifier: -