English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum

Patenge, N., Berendes, A., Engelhardt, H., Schuster, S. C., & Oesterhelt, D. (2001). The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum. Molecular Microbiology, 41(3), 653-663.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : Mol. Microbiol

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Patenge, N., Author
Berendes, A., Author
Engelhardt, H.1, Author              
Schuster, S. C.1, Author              
Oesterhelt, D.1, Author              
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: Complete genome sequence; Pseudomonas-aeruginosa; Twitching motility; Myxococcus-xanthus; Methanococcus-voltae; Escherichia-coli; Multigene family; Type-4 fimbriae; Proteins; Archaeon.; Microbiology in Current Contents(R)/Life Sciences.
 Abstract: In this study, a flagella-related protein gene cluster is described for Halobacterium salinarum. The fla gene cluster is located upstream of the flagellin genes flgB1-3 and oriented in the opposite direction. It consists of nine open reading frames (ORFs): htpIX, a member of the halobacterial transducer protein gene family, and the genes flaD-K. The genes flaD, E, G, H, I and J share high homologies with genes from other Archaea. Interestingly, flaK shows similarities to bacterial genes involved in the regulation of flagellar synthesis. The ORFs of flaH, flal and flaK contain sequences coding for nucleotide binding sites. Furthermore, flal contains a motif called the bacterial type II secretion protein E signature, indicating a functional relation to members of the bacterial pili type IV-type II secretion protein superfamily. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the genes flaE to flaK are transcribed into one polycistronic message. In frame deletion mutants of flal were generated by gene replacement. The deletion strain lacks motility and belongs to the fla(-) mutant class, indicating that it is deficient in flagellar biogenesis. The overall amount of flagellin protein in Delta flal cells is reduced, although transcription of the flagellin genes is unaffected. Therefore, the flal gene product is involved in the biosynthesis, transport or assembly of flagella in H. salinarum. [References: 62]

Details

show
hide
Language(s):
 Dates: 2001
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 318626
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Microbiology
  Alternative Title : Mol. Microbiol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 41 (3) Sequence Number: - Start / End Page: 653 - 663 Identifier: -