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  The TOM core complex: The general protein import pore of the outer membrane of mitochondria

Ahting, U., Thun, C., Hegerl, R., Typke, D., Nargang, F. E., Neupert, W., et al. (1999). The TOM core complex: The general protein import pore of the outer membrane of mitochondria. Journal of Cell Biology, 147(5), 959-968.

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Genre: Journal Article
Alternative Title : J. Cell Biol

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Ahting, U., Author
Thun, C., Author
Hegerl, R.1, Author              
Typke, D.1, Author              
Nargang, F. E., Author
Neupert, W.1, Author              
Nussberger, S., Author
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1External Organizations, ou_persistent22              

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Free keywords: Tom complex; Mitochondria; Protein translocation channel; Electron tomography; Protein targeting.; Intermembrane-space domain; Receptor complex; Precursor proteins; Preprotein translocase; Endoplasmic-reticulum; Targeting sequences; Insertion pore; Yeast mas17; Channel; Mom22.; Cell & Developmental Biology in Current Contents(R)/Life Sciences.
 Abstract: Translocation of nuclear-encoded preproteins across the outer membrane of mitochondria is mediated by the multicomponent transmembrane TOM complex. We have isolated the TOM core complex of Neurospora crassa by removing the receptors Tom70 and Tom20 from the isolated TOM holo complex by treatment with the detergent dodecyl maltoside. It consists of Tom40, Tom22, and the small Tom components, Tom6 and Tom7. This core complex was also purified directly from mitochondria after solubilization with dodecyl maltoside. The TOM core complex has the characteristics of the general insertion pore; it contains high-conductance channels and binds preprotein in a targeting sequence-dependent manner. It forms a double ring structure that, in contrast to the hole complex, lacks the third density seen in the latter particles. Three-dimensional reconstruction by electron tomography exhibits two open pores traversing the complex with a diameter of similar to 2.1 nm and a height of similar to 7 nm. Tom40 is the key structural element of the TOM core complex. [References: 60]

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 Dates: 1999
 Publication Status: Published in print
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 Identifiers: eDoc: 318472
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Title: Journal of Cell Biology
  Alternative Title : J. Cell Biol
Source Genre: Journal
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Pages: - Volume / Issue: 147 (5) Sequence Number: - Start / End Page: 959 - 968 Identifier: -