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  Nonlinear and asymmetric open channel characteristics of an ion-selective porin in planar membranes

Mathes, A., & Engelhardt, H. (1998). Nonlinear and asymmetric open channel characteristics of an ion-selective porin in planar membranes. Biophysical Journal, 75(3), 1255-1262.

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Mathes, A., Author
Engelhardt, H.1, Author           
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1External Organizations, ou_persistent22              

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Free keywords: Escherichia-coli porins; Lipid bilayer-membranes; Outer-membrane; Acidovorax-delafieldii; Functional-characterization; Rhodopseudomonas-blastica; Paracoccus-denitrificans; Electrostatic properties; Electrical properties; One conformation.; Biochemistry & biophysics.
 Abstract: The open channel characteristics of the bacterial porin Omp32 from Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (less than or equal to 30 mM KCI) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins. [References: 44]

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 Dates: 1998-09
 Publication Status: Published in print
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 Identifiers: eDoc: 318654
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Title: Biophysical Journal
Source Genre: Journal
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Pages: - Volume / Issue: 75 (3) Sequence Number: - Start / End Page: 1255 - 1262 Identifier: -