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  The regulated outer membrane protein omp21 from comamonas acidovorans is identified as a member of a new family of eight-stranded beta-sheet proteins by its sequence and properties

Baldermann, C., Lupas, A., Lubieniecki, J., & Engelhardt, H. (1998). The regulated outer membrane protein omp21 from comamonas acidovorans is identified as a member of a new family of eight-stranded beta-sheet proteins by its sequence and properties. Journal of Bacteriology, 180(15), 3741-3749.

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Baldermann, C., Author
Lupas, A.1, Author              
Lubieniecki, J., Author
Engelhardt, H.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Escherichia-coli; Nucleotide-sequence; Molecular characterization; Pseudomonas-acidovorans; 3-dimensional structure; Polyacrylamide gels; Secondary structure; Surface protein; Dna-sequence; Silver stain.; Microbiology.
 Abstract: Omp21, a minor outer membrane protein of the soil bacterium Comnmonas acidovorans, was purified from a spontaneous mutant lacking a surface layer and long-chain lipopolysaccharide, Omp21 synthesis is enhanced by oxygen depletion, and the protein has a variable electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis due to its heat-modifiable behavior. The structural gene omp21 encodes a precursor of 204 amino acids with a putative signal peptide of 21 amino acids. Mature Omp21 is a typical outer membrane protein with a high content of beta structure as determined by infrared spectroscopy. Sequence comparisons show that it belongs to a new outer membrane protein family, characterized by eight amphipathic beta strands, which includes virulence proteins, such as the neisserial opacity proteins, Salmonella typhimurium Rck, and Yersinia enterocolitica Ail, as well as the major outer membrane proteins OmpA from Escherichia coli and OprF from Pseudomonas aeruginosa. [References: 71]

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 Dates: 1998-08
 Publication Status: Published in print
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 Identifiers: eDoc: 318466
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Title: Journal of Bacteriology
Source Genre: Journal
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Pages: - Volume / Issue: 180 (15) Sequence Number: - Start / End Page: 3741 - 3749 Identifier: -