English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  26s proteasome structure revealed by three-dimensional electron microscopy

Walz, J., Erdmann, A., Kania, M., Typke, D., Koster, A. J., & Baumeister, W. (1998). 26s proteasome structure revealed by three-dimensional electron microscopy. Journal of Structural Biology, 121(1), 19-29.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Walz, J.1, Author              
Erdmann, A., Author
Kania, M., Author
Typke, D.1, Author              
Koster, A. J.1, Author              
Baumeister, W.1, Author              
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: 20s proteasome; Complex; Tomography.; Biochemistry & biophysics.
 Abstract: In 26S proteasomes, ''19S cap complexes'' associate with either one or both ends of the barrel-shaped 20S core complex. These regulatory complexes which comprise about 20 different subunits, including 6 ATPases of the AAA family, are thought to recognize ubiquitinated substrate proteins, to dissociate and unfold them before threading them into the 20S core where they are degraded. Here, we examine the structure of 26S proteasomes from Drosophila embryos and Xenopus oocytes by electron microscopy. Image analysis reveals a rather flexible linkage between the 19S caps and the 20S core, with a peculiar wagging-type movement of the caps relative to the core. At this stage of the analysis, it is not clear whether this movement is relevant in terms of function. Three-dimensional reconstructions, taking this into account, provide first insights into the remarkably complex structure of the 19S caps and allows us to put forward a composite model of the entire 26S complex. (C) 1998 Academic Press. [References: 28]

Details

show
hide
Language(s):
 Dates: 1998-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 318646
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Structural Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 121 (1) Sequence Number: - Start / End Page: 19 - 29 Identifier: -