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  Electron tomography of single ice-embedded macromolecules - three-dimensional alignment and classification

Walz, J., Typke, D., Nitsch, M., Koster, A. J., Hegerl, R., & Baumeister, W. (1997). Electron tomography of single ice-embedded macromolecules - three-dimensional alignment and classification. Journal of Structural Biology, 120(3), 387-395.

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Walz, J.1, Author              
Typke, D.1, Author              
Nitsch, M., Author
Koster, A. J.1, Author              
Hegerl, R.1, Author              
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Escherichia-coli; Biological particles; Ribosome; Reconstruction; Micrographs; Microscopy; Resolution; Images.; Biochemistry & biophysics.
 Abstract: From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level. (C) 1997 Academic Press. [References: 24]

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 Dates: 1997-12
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: eDoc: 318718
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Title: Journal of Structural Biology
Source Genre: Journal
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Pages: - Volume / Issue: 120 (3) Sequence Number: - Start / End Page: 387 - 395 Identifier: -