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  The thermosome - alternating alpha and beta-subunits within the chaperonin of the archaeon thermoplasma acidophilum

Nitsch, M., Klumpp, M., Lupas, A., & Baumeister, W. (1997). The thermosome - alternating alpha and beta-subunits within the chaperonin of the archaeon thermoplasma acidophilum. Journal of Molecular Biology, 267(1), 142-149.

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Nitsch, M., Author
Klumpp, M., Author
Lupas, A.1, Author              
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Chaperone; Thermosome; Heat shock; Electron cryo-microscopy; Archaea.; T-complex polypeptide-1; Molecular chaperone; Thermophilic archaebacterium; Cytoplasmic chaperonin; Cytosolic chaperonin; Electron-microscopy; Eukaryotic cytosol; Escherichia-coli; Central cavity; Heat-shock.; Molecular biology & genetics.
 Abstract: The thermosome of the archaeon Thermoplasma acidophilum is composed of two subunits, alpha and beta, which are arranged in two stacked, eight-membered rings. Electron cryo-microscopy in conjunction with image analysis revealed a 4-fold symmetry in the heterooligomeric alpha + beta thermosome isolated from Thermoplasma, but not in the homooligomeric alpha-only thermosome expressed in Escherichia coli. This indicates that alpha and P-subunits alternate within the rings of the Thermoplasma thermosome rather than forming two different homooligomeric rings. In addition, a small subpopulation of 9-fold symmetric complexes was found among the recombinant alpha-only thermosomes, and a central mass most likely representing bound substrate molecules was observed in about half of the native and recombinant thermosome particles. (C) 1997 Academic Press Limited. [References: 36]

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 Dates: 1997-03-21
 Publication Status: Published in print
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 Identifiers: eDoc: 318543
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Title: Journal of Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 267 (1) Sequence Number: - Start / End Page: 142 - 149 Identifier: -