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  Subunit topology of the rhodococcus proteasome

Zühl, F., Tamura, T., Dolenc, I., Cejka, Z., Nagy, I., Demot, R., et al. (1997). Subunit topology of the rhodococcus proteasome. FEBS Letters, 400(1), 83-90.

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Zühl, F.1, Author              
Tamura, T., Author
Dolenc, I., Author
Cejka, Z., Author
Nagy, I.1, Author              
Demot, R., Author
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Proteasome; Proteolysis; Rhodococcus; Gene expression.; Multicatalytic proteinase prosome; Thermoplasma-acidophilum; Structural features; Electrophoresis; Complex; Images.; Biochemistry & biophysics.
 Abstract: The 20S proteasome, isolated from the nocardioform actinomycete Rhodococcus erythropolis strain NI86/21, is built from two alpha-type and two beta-type subunits. In order to probe the subunit topology, we have set up an expression system which allows coexpression of the genes encoding the alpha- and beta-subunits in all. possible combinations. The four respective constructs obtained yielded fully assembled and proteolytically active proteasomes. Biochemical, kinetic and electron microscopy analysis allow us to rule out several of the models which were originally envisaged for the subunit topology of the Rhodococcus proteasome. The experiments further indicate that the assembly pathways of the Rhodococcus and of the Thermoplasma proteasome differ in some important details. [References: 30]

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 Dates: 1997-01-02
 Publication Status: Published in print
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 Identifiers: eDoc: 318377
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 400 (1) Sequence Number: - Start / End Page: 83 - 90 Identifier: -