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  Zero-loss energy filtering under low-dose conditions using a post-column energy filter

Grimm, R., Koster, A. J., Ziese, U., Typke, D., & Baumeister, W. (1996). Zero-loss energy filtering under low-dose conditions using a post-column energy filter. Journal of Microscopy, 183(Part 1), 60-68.

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Grimm, R., Author
Koster, A. J.1, Author              
Ziese, U., Author
Typke, D.1, Author              
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Eftem; Electron cryomicroscopy; Energy filtering; Fourier ring correlation; Low dose; Thermoplasma; Tmv; Vesicle; Zero-loss filtering.; Automatic electron tomography.; Microbiology.
 Abstract: Electron cryomicroscopy combined with energy filtering can be performed under low-dose conditions using a post-column energy filter. The microscope combined with the filter is set up such that it can be used with similar ease as a conventional microscope, the main difference being that all filter and microscope control is performed through a central computer and images are recorded with a cooled slow-scan CCD camera. The microscope can also still be used for regular imaging on film as without the filter. Owing to the 18 times post-magnification of the filter, the microscope normally has to be operated at a small magnifications, e.g. 3000x, and the beam has to be contracted to a small spot, e.g. 5 mm, in the plane of the microscope viewing screen. Computer control allows one to perform a variety of tasks automatically, such as autofocusing, thickness measurements, most-probable-loss imaging. CCD spot-scanning and tonography. The gain in contrast due to zero-loss energy filtering is analysed using visual inspection, power spectra and Fourier ring correlation. The thickness range for ice-embedded specimens in which a filter at 120 kV is most useful appears to be between 100 and 300 nm. [References: 12]

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 Dates: 1996-07
 Publication Status: Published in print
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 Identifiers: eDoc: 318705
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Title: Journal of Microscopy
Source Genre: Journal
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Pages: - Volume / Issue: 183 (Part 1) Sequence Number: - Start / End Page: 60 - 68 Identifier: -