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キーワード:
Thermosome; Molecular chaperone; Gene expression; Archaebacteria; Thermoplasma acidophilum; Electron microscopy.; Thermophilic archaebacterium; Escherichia-coli; Rna-polymerase; Polypeptide-1; Subunits; System; Genes.; Biochemistry & biophysics.
要旨:
Go-expression of the two genes encoding the alpha- and beta-subunit of the Thermoplasma acidophilum thermosome in Escher ichia coli yielded fully assembled hetero-oligomeric complexes (alpha + beta). Surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (alpha, beta) in the bacterial cytoplasm. On electron micrographs these complexes were indistinguishable from each other and from the native thermosome. The recombinant alpha-complex as well as the native thermosome could be reconstituted in vitro from their dissociated subunits in the presence of Mg-ATP. [References: 28]
