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  Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomography

Dierksen, K., Typke, D., Hegerl, R., Walz, J., Sackmann, E., & Baumeister, W. (1995). Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomography. Biophysical Journal, 68(4), 1416-1422.

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Dierksen, K., Author
Typke, D.1, Author              
Hegerl, R.1, Author              
Walz, J.1, Author              
Sackmann, E., Author
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Microscopy; Membranes; System.; Biochemistry & biophysics.
 Abstract: Automated electron tomography is shown to be a suitable means to Visualize the shape of phospholipid vesicles embedded in vitrified ice. With a slow-scan charge-coupled device camera as a recording device, the cumulative electron dose needed to record a data set of 60 projections at a magnification of 20,000x can be kept as low as 15 e(-)/Angstrom(2) (or 1500 electrons/nm(2)). The membrane of the three-dimensionally reconstructed vesicles is clearly Visible in two-dimensional sections through the three-dimensionally reconstructed volume. Some edges indicating a polygonal shape of the vesicles, frozen from the gel phase, are also clearly recognized. Because of the presently limited tilt angle range (+/-60 degrees), the upper and lower ''caps'' of the vesicles (representing about 35% of the surface of the ellipsoidal particles) remain invisible in the three-dimensional reconstruction. [References: 18]

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 Dates: 1995-04
 Publication Status: Published in print
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 Identifiers: eDoc: 318455
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Title: Biophysical Journal
Source Genre: Journal
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Pages: - Volume / Issue: 68 (4) Sequence Number: - Start / End Page: 1416 - 1422 Identifier: -