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  The thermosome of thermoplasma acidophilum and its relationship to the eukaryotic chaperonin tric

Waldmann, T., Nimmesgern, E., Nitsch, M., Peters, J., Pfeifer, G., Müller, S., et al. (1995). The thermosome of thermoplasma acidophilum and its relationship to the eukaryotic chaperonin tric. European Journal of Biochemistry, 227(3), 848-856.

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Waldmann, T., Author
Nimmesgern, E., Author
Nitsch, M., Author
Peters, J.1, Author              
Pfeifer, G.1, Author              
Müller, S.1, Author              
Kellermann, J.1, Author              
Engel, A., Author
Hartl, F. U.1, Author              
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Thermosome; Tcp1 ring complex; Chaperonin; Thermoplasma acidophilum; Archaebacteria.; T-complex polypeptide-1; Transmission electron-microscopy; Molecular chaperone; Heat-shock; Thermophilic archaebacterium; In-vivo; Proteins; Groel; Cell; Proteasomes.; Biochemistry & biophysics.
 Abstract: A high molecular-mass protein complex from the archaebacterium Thermoplasma acidophilum, referred to here as the 'thermosome', is built from two subunits (M(r)58 and 60). The thermosome has been purified to homogeneity. The molecular mass of the native complex was determined to be 1061 +/- 30 Da by scanning transmission electron microscopy. It shows a weak ATPase activity and is able to bind denatured polypeptides. Averages obtained from electron micrographs of negatively stained molecules in the end-on and side-on orientations, respectively were compared with these of the t-complex polypeptide 1 ring complex (TRiC), isolated from bovine testes. Both molecules consist of two stacked pseudo eightfold symmetric rings which build up a cylindrical particle with a large cavity in the center. Sequence alignments of peptides generated from both subunits of the thermosome and different subunits of TRiC reveal a high partial similarity to each other and to the archaebacterial chaperonin thermophilic factor 55 from Sulfolobus shibatae as well as to eukaryotic TCP1 proteins. These striking structural similarities confirm the proposition that all these molecules belong to a single protein family which is structurally and functionally related to the GroEL class of molecular chaperones. [References: 48]

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 Dates: 1995-02-01
 Publication Status: Published in print
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 Identifiers: eDoc: 318511
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Title: European Journal of Biochemistry
Source Genre: Journal
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Pages: - Volume / Issue: 227 (3) Sequence Number: - Start / End Page: 848 - 856 Identifier: -