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  Three-dimensional structure of the surface protein layer (MW layer) of Bacillus brevis 47

Tsuboi, A., Engelhardt, H., Santarius, U., Tsukagoshi, N., Udaka, S., & Baumeister, W. (1989). Three-dimensional structure of the surface protein layer (MW layer) of Bacillus brevis 47. Journal of Ultrastructure & Molecular Structure Research., 102(2), 178-187.

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Tsuboi, A., Author
Engelhardt, H.1, Author              
Santarius, U.1, Author              
Tsukagoshi, N., Author
Udaka, S., Author
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: *Bacillus/ul [Ultrastructure]; Cell Membrane/ul [Ultrastructure]; Microscopy, Electron; Support, Non-U.S. Gov't
 Abstract: The three-dimensional (3D) structure of one surface protein layer from Bacillus brevis 47, the middle wall (MW) layer, has been reconstructed from tilted-view electron micrographs after correlation averaging to a resolution of 2 nm. The MW layer has p6 symmetry with a center-to-center spacing of 18.3 nm and a minimum thickness of 5.5 nm. The reconstruction reveals a distinct domain structure: the heavier domain of six monomers jointly forms a massive core centered at the sixfold symmetry axis, and lighter domains interconnect adjacent unit cells. In addition, the larger domains collectively form a pore by making contact with each other towards the inner surface, while the smaller domains establish a second connectivity towards the outer surface of the S layer. The MW layer of B. brevis resembles the S layer of Acetogenium kivui in various aspects: they have very similar lattice parameters and highly reminiscent 3D structures; the pores penetrate through the whole core and appear to determine the porosity of the S layers.

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 Dates: 1989
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: eDoc: 318333
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Title: Journal of Ultrastructure & Molecular Structure Research.
Source Genre: Journal
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Pages: - Volume / Issue: 102 (2) Sequence Number: - Start / End Page: 178 - 187 Identifier: -