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  Three-dimensional reconstruction of a freeze-dried and metal-shadowed bacterial surface layer

Wildhaber, I., Hegerl, R., Barth, M., Gross, H., & Baumeister, W. (1986). Three-dimensional reconstruction of a freeze-dried and metal-shadowed bacterial surface layer. Ultramicroscopy, 19(1), 57-68.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-74F6-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-74F7-2
Genre: Journal Article

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Wildhaber, I., Author
Hegerl, R.1, Author              
Barth, M., Author
Gross, H., Author
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Electron microscope examination of materials; Molecular biophysics; Proteins; Biomolecular structure, configuration, conformation, and active sites [A8715B]
 Abstract: Three-dimensional reconstructions were performed from tilt series of a rotary-shadowed regular surface layer. Two series, representing the two surfaces of the HPI layer, were processed. The metal film stands out very clearly from the surrounding matter (protein, carbon) in the reconstructions and can, in good approximation, be regarded as reproducing the surface geometry of the protein layer. Thus, by combining information from both surfaces, it is possible to reconstruct profiles of the protein structure. Shadowing is advantageous in that it provides excellent reproducibility in specimen contrast, and the metal film is stable in the electron beam. Moreover, unlike negative staining, information is obtained from surfaces that are not in contact with the specimen support, and the technique is also applicable to freeze-dried material. (21 References).

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 Dates: 1986
 Publication Status: Published in print
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 Identifiers: eDoc: 318531
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Title: Ultramicroscopy
Source Genre: Journal
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Pages: - Volume / Issue: 19 (1) Sequence Number: - Start / End Page: 57 - 68 Identifier: -