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  Three-dimensional structure of the tetragonal surface layer of Sporosarcina ureae

Engelhardt, H., Saxton, W. O., & Baumeister, W. (1986). Three-dimensional structure of the tetragonal surface layer of Sporosarcina ureae. Journal of Bacteriology., 168(1), 309-317.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-74FE-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-74FF-1
Genre: Journal Article

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 Creators:
Engelhardt, H.1, Author              
Saxton, W. O.1, Author              
Baumeister, W.1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: Bacterial Proteins; *Gram-Positive Bacteria/ul [Ultrastructure]; Image Processing, Computer-Assisted; Microscopy, Electron; Protein Conformation; Spectrophotometry, Infrared
 Abstract: The three-dimensional structure of the regular surface layer of Sporosarcina ureae has been determined to a resolution of 1.7 nm by electron microscopy and image reconstruction. The S-layer has p4 symmetry, a lattice constant of 12.9 nm, and a minimum thickness of 6.6 nm. The reconstruction reveals a distinct domain structure: a massive core, arms connecting adjacent unit cells, and spurs which make contact at the subsidiary fourfold symmetry axes. In the z-direction the domains appear to be arranged in three planes, creating two entirely different surface reliefs. The S-layer has a complex pattern of pores and gaps that are 2 to 3 nm wide. In addition, the secondary-structure composition has been determined by infrared spectroscopy: about 35% of the polypeptide appears to have a beta-structure conformation.

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 Dates: 1986
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: eDoc: 318750
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Title: Journal of Bacteriology.
Source Genre: Journal
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Pages: - Volume / Issue: 168 (1) Sequence Number: - Start / End Page: 309 - 317 Identifier: -