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  Alterations of pre-mRNA splicing in human inflammatory bowel disease

Hasler, R., Kerick, M., Mah, N., Hultschig, C., Richter, G., Bretz, F., et al. (2011). Alterations of pre-mRNA splicing in human inflammatory bowel disease. European Journal of Cell Biology, 90(6-7), 603-11. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/21324547 http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=273240&_user=28761&_pii=S0171933510002608&_check=y&_origin=article&_zone=toolbar&_coverDate=31-Jul-2011&view=c&originContentFamily=serial&wchp=dGLzVlB-zSkzS&md5=fdf9d076a3f52267e1e46408d05f30bc/1-s2.0-S0171933510002608-main.pdf http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=273240&_user=28761&_pii=S0171933510002608&_check=y&_origin=article&_zone=toolbar&_coverDate=31-Jul-2011&view=c&originContentFamily=serial&wchp=dGLbVBA-zSkWz&md5=fdf9d076a3f52267e1e46408d05f30bc/1-s2.0-S0171933510002608-main.pdf.

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 Creators:
Hasler, R., Author
Kerick, M.1, Author              
Mah, N., Author
Hultschig, C., Author
Richter, G., Author
Bretz, F., Author
Sina, C., Author
Lehrach, H.2, Author              
Nietfeld, W.2, Author              
Schreiber, S., Author
Rosenstiel, P., Author
Affiliations:
1Cancer Genomics (Michal-Ruth Schweiger), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479649              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Animals; Colitis, Ulcerative/genetics/metabolism/pathology; Crohn Disease/genetics/metabolism/pathology; Humans; Inflammatory Bowel Diseases/*genetics/metabolism/pathology; Mice; Mice, Knockout; RNA Precursors/*genetics/metabolism; RNA Splicing
 Abstract: Alternative pre-mRNA splicing is regarded as a pivotal mechanism for generating proteome diversity and complexity from a limited inventory of mammalian genes. Aberrant splicing has been described as a predisposing factor for a number of diseases, but very little is known about its role in chronic inflammation. In this study, we systematically screened 149 splicing factors and 145 potential intron retention events for occurrence and differential expression in inflammatory bowel diseases (IBD). As a result, we identified 47 splicing factors and 33 intron retention events that were differentially regulated in mucosal tissue of IBD patients at transcript level. Despite the fact that Crohn's disease and ulcerative colitis, two subtypes of IBD, share the expression patterns of splicing factors and intron retention events in the majority of cases, we observed significant differences. To investigate these subtype-specific changes in detail we determined the expression levels of seven splicing factors (DUSP11, HNRPAB, HNRPH3, SLU7, SFR2IP, SFPQ, SF3B14) and three intron retention events (PARC, IER3, FGD2) in a cohort of 165 patients with inflammatory diseases of the colon (120 with IBD) and 30 healthy controls by real time PCR (TaqMan). This study demonstrates the potential impact of regulated splicing factors on subsequent regulated intron retention in the pathogenesis of chronic inflammation, exemplified by IBD.

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Title: European Journal of Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 90 (6-7) Sequence Number: - Start / End Page: 603 - 11 Identifier: ISSN: 1618-1298 (Electronic) 0171-9335 (Linking)