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  A streamlined protocol for emulsion polymerase chain reaction and subsequent purification

Schutze, T., Rubelt, F., Repkow, J., Greiner, N., Erdmann, V. A., Lehrach, H., et al. (2011). A streamlined protocol for emulsion polymerase chain reaction and subsequent purification. Analytical Biochemistry, 410(1), 155-157. doi:10.1016/j.ab.2010.11.029.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-787B-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-787D-9
Genre: Journal Article

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 Creators:
Schutze, T.1, Author              
Rubelt, F.2, Author              
Repkow, J.1, Author              
Greiner, N.1, Author              
Erdmann, V. A., Author
Lehrach, H.1, Author              
Konthur, Z.2, Author              
Glokler, J.1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479653              

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Free keywords: Chemical Fractionation/*methods; DNA/*genetics/*isolation & purification; Emulsions; Humans; Oils/*chemistry; Polymerase Chain Reaction/*methods; Time Factors; Water/*chemistry
 Abstract: Compartmentalization of polymerase chain reaction (PCR) reduces artifacts, especially when complex libraries are amplified. It allows clonal amplification of templates from complex mixtures in a bias-free manner. Here we describe a rapid, straightforward, and easy protocol for PCR in a water-in-oil emulsion (ePCR) including sample recovery by DNA purification. Furthermore, no special laboratory equipment is needed and inexpensive components are used. Therefore, our flexible protocol allows ePCR to be readily implemented in daily routine experiments for a broad range of applications.

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 Dates: 2011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 584755
DOI: 10.1016/j.ab.2010.11.029
 Degree: -

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Title: Analytical Biochemistry
Source Genre: Journal
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Pages: - Volume / Issue: 410 (1) Sequence Number: - Start / End Page: 155 - 157 Identifier: ISSN: 1096-0309 (Electronic) 0003-2697 (Print)