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  Design and Screening of M13 Phage Display cDNA Libraries

Georgieva, Y., & Konthur, Z. (2011). Design and Screening of M13 Phage Display cDNA Libraries. Molecules, 16(2), 1667-1681. Retrieved from <Go to ISI>://000287745400044 http://www.mdpi.com/1420-3049/16/2/1667/pdf.

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 Creators:
Georgieva, Y.1, Author              
Konthur, Z.1, Author              
Affiliations:
1In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479653              

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Free keywords: phage display; cdna library; orf selection; phagemid; protein-protein interaction; next generation sequencing (ngs); open reading frames; antibody variable domains; fd adsorption protein; filamentous phage; escherichia-coli; surface expression; functional cloning; bacteriophage fd; helper phage; selection
 Abstract: The last decade has seen a steady increase in screening of cDNA expression product libraries displayed on the surface of filamentous bacteriophage. At the same time, the range of applications extended from the identification of novel allergens over disease markers to protein-protein interaction studies. However, the generation and selection of cDNA phage display libraries is subjected to intrinsic biological limitations due to their complex nature and heterogeneity, as well as technical difficulties regarding protein presentation on the phage surface. Here, we review the latest developments in this field, discuss a number of strategies and improvements anticipated to overcome these challenges making cDNA and open reading frame (ORF) libraries more readily accessible for phage display. Furthermore, future trends combining phage display with next generation sequencing (NGS) will be presented.

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 Dates: 2011
 Publication Status: Published in print
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 Identifiers: eDoc: 584689
URI: ://000287745400044 http://www.mdpi.com/1420-3049/16/2/1667/pdf
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Title: Molecules
Source Genre: Journal
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Pages: - Volume / Issue: 16 (2) Sequence Number: - Start / End Page: 1667 - 1681 Identifier: ISSN: 1420-3049