English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Induction and Selection of Sox17-Expressing Endoderm Cells Generated from Murine Embryonic Stem Cells

Schroeder, I. S., Sulzbacher, S., Nolden, T., Fuchs, J., Czarnota, J., Meisterfeld, R., et al. (2011). Induction and Selection of Sox17-Expressing Endoderm Cells Generated from Murine Embryonic Stem Cells. Cells, Tissues, Organs. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/22123608 http://content.karger.com/ProdukteDB/produkte.asp?Aktion=ShowPDF&ArtikelNr=000329864&ProduktNr=224197&filename=000329864.pdf http://content.karger.com/ProdukteDB/produkte.asp?Aktion=ShowPDF&ArtikelNr=000329864&Ausgabe=256901&ProduktNr=224197&filename=000329864.pdf.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Schroeder, I. S., Author
Sulzbacher, S., Author
Nolden, T., Author
Fuchs, J., Author
Czarnota, J., Author
Meisterfeld, R., Author
Himmelbauer, H.1, Author              
Wobus, A. M., Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Content

show
hide
Free keywords: -
 Abstract: Embryonic stem (ES) cells offer a valuable source for generating insulin-producing cells. However, current differentiation protocols often result in heterogeneous cell populations of various developmental stages. Here we show the activin A-induced differentiation of mouse ES cells carrying a homologous dsRed-IRES-puromycin knock-in within the Sox17 locus into the endoderm lineage. Sox17-expressing cells were selected by fluorescence-assisted cell sorting (FACS) and characterized at the transcript and protein level. Treatment of ES cells with high concentrations of activin A for 10 days resulted in up to 19% Sox17-positive cells selected by FACS. Isolated Sox17-positive cells were characterized by defini- tive endoderm-specific Sox17/Cxcr4/Foxa2 transcripts, but lacked pluripotency-associated Oct4 mRNA and protein. The Sox17-expressing cells showed downregulation of extraembryonic endoderm (Sox7, Afp, Sdf1)-, mesoderm (Foxf1, Meox1)- and ectoderm (Pax6, NeuroD6)-specific transcripts. The presence of Hnf4alpha, Hes1 and Pdx1 mRNA demonstrated the expression of primitive gut/foregut cell-specific markers. Ngn3, Nkx6.1 and Nkx2.2 transcripts in Sox17-positive cells were determined as properties of pancreatic endocrine progenitors. Immunocytochemistry of activin A-induced Sox17-positive embryoid bodies revealed coexpression of Cxcr4 and Foxa2. Moreover, the histochemical demonstration of E-cadherin-, Cxcr4-, Sox9-, Hnf1beta- and Ngn3-positive epithelial-like structures underlined the potential of Sox17-positive cells to further differentiate into the pancreatic lineage. By reducing the heterogeneity of the ES cell progeny, Sox17-expressing cells are a suitable model to evaluate the effects of growth and differentiation factors and of culture conditions to delineate the differentiation process for the generation of pancreatic cells in vitro.

Details

show

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cells, Tissues, Organs
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1422-6421 (Electronic) 1422-6405 (Linking)