English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Prospectively isolated CD133/CD24-positive ependymal cells from the adult spinal cord and lateral ventricle wall differ in their long-term in vitro self-renewal and in vivo gene expression

Pfenninger, C. V., Steinhoff, C., Hertwig, F., & Nuber, U. A. (2011). Prospectively isolated CD133/CD24-positive ependymal cells from the adult spinal cord and lateral ventricle wall differ in their long-term in vitro self-renewal and in vivo gene expression. GLIA, 59(1), 68-81. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21046556 http://onlinelibrary.wiley.com/store/10.1002/glia.21077/asset/21077_ftp.pdf?v=1&t=gyzpsilx&s=2043b8bd470bb80e510728f41471ffeca3959b12.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Pfenninger, C. V., Author
Steinhoff, C.1, Author           
Hertwig, F., Author
Nuber, U. A.2, Author           
Affiliations:
1Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

Content

show
hide
Free keywords: Animals; Antigens, CD/*metabolism; Antigens, CD24/*metabolism; Cell Differentiation; Cells, Cultured; Ependyma/*cytology/metabolism; Flow Cytometry; *Gene Expression; Glycoproteins/*metabolism; Immunohistochemistry; Lateral Ventricles/*cytology/metabolism; Mice; Neural Stem Cells/cytology/metabolism; Oligonucleotide Array Sequence Analysis; Peptides/*metabolism; Reverse Transcriptase Polymerase Chain Reaction; Spinal Cord/*cytology/metabolism
 Abstract: In contrast to ependymal cells located above the subventricular zone (SVZ) of the adult lateral ventricle wall (LVW), adult spinal cord (SC) ependymal cells possess certain neural stem cell characteristics. The molecular basis of this difference is unknown. In this study, antibodies against multiple cell surface markers were applied to isolate pure populations of SC and LVW ependymal cells, which allowed a direct comparison of their in vitro behavior and in vivo gene expression profile. Isolated CD133(+)/CD24(+)/CD45(-)/CD34(-) ependymal cells from the SC displayed in vitro self-renewal and differentiation capacity, whereas those from the LVW did not. SC ependymal cells showed a higher expression of several genes involved in cell division, cell cycle regulation, and chromosome stability, which is consistent with a long-term self-renewal capacity, and shared certain transcripts with neural stem cells of the embryonic forebrain. They also expressed several retinoic acid (RA)-regulated genes and responded to RA exposure. LVW ependymal cells showed higher transcript levels of many genes regulated by transforming growth factor-beta family members. Among them were Dlx2, Id2, Hey1, which together with Foxg1 could explain their potential to turn into neuroblasts under certain environmental conditions.

Details

show
hide
Language(s):
 Dates: 2011
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: GLIA
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 59 (1) Sequence Number: - Start / End Page: 68 - 81 Identifier: ISSN: 1098-1136 (Electronic) 0894-1491 (Linking)