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  Live cell imaging of meiotic chromosome dynamics in yeast

Scherthan, H., & Adelfalk, C. (2011). Live cell imaging of meiotic chromosome dynamics in yeast. Methods Mol Biol, 745, 537-48. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21660715 http://www.springerlink.com/content/xt4w7xkv720wr512/fulltext.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7973-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7974-4
Genre: Journal Article

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 Creators:
Scherthan, H.1, Author              
Adelfalk, C.1, Author              
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: Chromosomes, Fungal/*genetics; DNA Breaks, Double-Stranded; Meiosis/*genetics; Saccharomyces cerevisiae/*genetics
 Abstract: Recombination in first meiotic prophase is initiated by endogenous breaks in double-stranded DNA (DSBs) which occurs during a time when chromosomes are remodeled and proteinaceous cores (axes) are assembled along their length. DSBs are instrumental in homologue recognition and underlie the crossovers that form between parental chromosomes to ensure genome haploidization during the following two successive meiotic divisions. Advances in fluorescence microscopy and genetic engineering of GFP-tagged fusion proteins have made it possible to observe the behavior of entire chromosomes and specific subregions in live cells of the yeast Saccharomyces cerevisiae. In meiosis we observed that telomeres are dynamic and move about the entire nuclear periphery, only interrupted by their fleeting clustering at the spindle pole body (the centrosome equivalent), known as bouquet formation. This mobility translates to whole chromosomes and nuclei during the entire prophase I. Here we describe a simple setup for live cell microscopy that we used to observe chromosome movements during a time when DSBs are formed and transform into crossover and non-crossover products.

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 Dates: 2011
 Publication Status: Published in print
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Title: Methods Mol Biol
Source Genre: Journal
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Pages: - Volume / Issue: 745 Sequence Number: - Start / End Page: 537 - 48 Identifier: ISSN: 1940-6029 (Electronic) 1064-3745 (Linking)