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  Methylation at global LINE-1 repeats in human blood are affected by gender but not by age or natural hormone cycles

El-Maarri, O., Walier, M., Behne, F., van Uum, J., Singer, H., Diaz-Lacava, A., et al. (2011). Methylation at global LINE-1 repeats in human blood are affected by gender but not by age or natural hormone cycles. PLoS ONE, 6(1), e16252-e16252. doi:10.1371/journal.pone.0016252.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7975-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7977-D
Genre: Journal Article

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El-Maarri, O., Author
Walier, M.1, Author              
Behne, F., Author
van Uum, J., Author
Singer, H., Author
Diaz-Lacava, A., Author
Nusgen, N., Author
Niemann, B., Author
Watzka, M., Author
Reinsberg, J., Author
van der Ven, H., Author
Wienker, T.2, Author              
Stoffel-Wagner, B., Author
Schwaab, R., Author
Oldenburg, J., Author
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              
2Clinical Genetics (Thomas F. Wienker), Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479643              

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Free keywords: Adolescent; Adult; Age Factors; Blood; Cell Line; DNA/*blood; *DNA Methylation; Female; Gonadal Steroid Hormones/analysis/physiology; Humans; Long Interspersed Nucleotide Elements/*genetics; Male; Middle Aged; Receptors, Estrogen/analysis/physiology; Sex Factors; Young Adult
 Abstract: Previously, we reported on inter-individual and gender specific variations of LINE-1 methylation in healthy individuals. In this study, we investigated whether this variability could be influenced by age or sex hormones in humans. To this end, we studied LINE-1 methylation in vivo in blood-derived DNA from individuals aged 18 to 64 years and from young healthy females at various hormone levels during the menstrual cycle. Our results show that no significant association with age was observed. However, the previously reported increase of LINE-1 methylation in males was reconfirmed. In females, although no correlation between LINE-1 or Alu methylation and hormone levels was observed, a significant stable individual specific level of methylation was noted. In vitro results largely confirmed these findings, as neither estrogen nor dihydrotestosterone affected LINE-1 or Alu methylation in Hek293T, HUVEC, or MDA-kb2 cell lines. In contrast, a decrease in methylation was observed in estrogen-treated T47-Kbluc cell lines strongly expressing estrogen receptor. The very low expression of estrogen receptor in blood cells could explain the observed insensitivity of methylation at LINE-1 to natural hormonal variations in females. In conclusion, neither natural cycle of hormones nor age has a detectable effect on the LINE-1 methylation in peripheral blood cells, while gender remains an important factor.

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 Dates: 2011
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: eDoc: 584814
DOI: 10.1371/journal.pone.0016252
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Title: PLoS ONE
Source Genre: Journal
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Pages: - Volume / Issue: 6 (1) Sequence Number: - Start / End Page: e16252 - e16252 Identifier: ISSN: 1932-6203 (Electronic)