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  Rapid proteomic remodeling of cardiac tissue caused by total body ionizing radiation

Azimzadeh, O., Scherthan, H., Sarioglu, H., Barjaktarovic, Z., Conrad, M., Vogt, A., et al. (2011). Rapid proteomic remodeling of cardiac tissue caused by total body ionizing radiation. Proteomics, 11(16), 3299-311. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21751382 http://onlinelibrary.wiley.com/store/10.1002/pmic.201100178/asset/3299_ftp.pdf?v=1&t=gywo0rnk&s=1ed555ed68bcff3ccbc73cd53e6d3cb4b9609584.

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Azimzadeh, O., Author
Scherthan, H.1, Author           
Sarioglu, H., Author
Barjaktarovic, Z., Author
Conrad, M., Author
Vogt, A., Author
Calzada-Wack, J., Author
Neff, F., Author
Aubele, M., Author
Buske, C., Author
Atkinson, M. J., Author
Tapio, S., Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: Animals; Blotting, Western; Cardiomyopathies/metabolism/pathology; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; Gamma Rays/*adverse effects; Heart/*radiation effects; Lipid Peroxidation/radiation effects; Mass Spectrometry; Mice; Mice, Inbred C57BL; Oxidative Stress; Protein Carbonylation/radiation effects; Protein Interaction Mapping; Proteins/*analysis/metabolism; Proteome/analysis/*radiation effects; Proteomics; Radiation Injuries, Experimental/metabolism/pathology; Reproducibility of Results; Ventricular Remodeling/radiation effects; Whole-Body Irradiation
 Abstract: Accidental nuclear scenarios lead to environmental contamination of unknown level. Immediate radiation-induced biological responses that trigger processes leading to adverse health effects decades later are not well understood. A comprehensive proteomic analysis provides a promising means to identify and quantify the initial damage after radiation exposure. Early changes in the cardiac tissue of C57BL/6 mice exposed to total body irradiation were studied, using a dose relevant to both intentional and accidental exposure (3 Gy gamma ray). Heart tissue protein lysates were analyzed 5 and 24 h after the exposure using isotope-coded protein labeling (ICPL) and 2-dimensional difference-in-gel-electrophoresis (2-D DIGE) proteomics approaches. The differentially expressed proteins were identified by LC-ESI-MS-MS. Both techniques showed similar functional groups of proteins to be involved in the initial injury. Pathway analyses indicated that total body irradiation immediately induced biological responses such as inflammation, antioxidative defense, and reorganization of structural proteins. Mitochondrial proteins represented the protein class most sensitive to ionizing radiation. The proteins involved in the initial damage processes map to several functional categories involving cardiotoxicity. This prompts us to propose that these early changes are indicative of the processes that lead to an increased risk of cardiovascular disease after radiation exposure.

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 Dates: 2011
 Publication Status: Published in print
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Title: Proteomics
Source Genre: Journal
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Pages: - Volume / Issue: 11 (16) Sequence Number: - Start / End Page: 3299 - 311 Identifier: ISSN: 1615-9861 (Electronic) 1615-9853 (Linking)