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  Monozygotic twins with neurofibromatosis type 1 (NF1) display differences in methylation of NF1 gene promoter elements, 5' untranslated region, exon and intron 1.

Harder, A., Titze, S., Herbst, L., Harder, T., Guse, K., Tinschert, S., et al. (2010). Monozygotic twins with neurofibromatosis type 1 (NF1) display differences in methylation of NF1 gene promoter elements, 5' untranslated region, exon and intron 1. Twin Research and Human Genetics: the Official Journal of the International Society for Twin Studies., 13(6), 582-594. doi:10.1375/twin.13.6.582.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-79DD-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-79DE-5
Genre: Journal Article
Alternative Title : Twin Res Hum Genet

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 Creators:
Harder, A., Author
Titze, S., Author
Herbst, L., Author
Harder, T., Author
Guse, K., Author
Tinschert, S.1, Author              
Kaufmann, D., Author
Rosenbaum, T., Author
Mautner, V. F., Author
Windt, E., Author
Wahlländer-Danek, U., Author
Wimmer, K., Author
Mundlos, S.1, Author              
Peters, H., Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              

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Free keywords: neurofibromatosis type 1; NF1; promoter; methylation; monozygotic twin pairs
 Abstract: Neurofibromatosis type 1 (NF1) is a common autosomal dominant disorder caused by heterozygotic inactivation of the NF1 tumor suppressor gene at 17q11.2. The associated phenotypes are highly variable, and modifying genes have been proposed to explain at least in part the intriguing expressivity. Given that haploinsufficiency of the NF1 gene product neurofibromin is responsible for some of the clinical manifestations, variations in expression of the wildtype NF1 allele might modify the phenotype. We therefore investigated epigenetic molecular modifications that could result in variable expression of the normal NF1 allele. To exclude confounding by DNA sequence variations, we analyzed monozygotic twin pairs with NF1 who presented with several discordant features. We fine-mapped the methylation pattern of a nearly 1 kb NF1 promoter region in lymphocytes of 8 twin pairs. All twin pairs showed significant intra-pair differences in methylation, especially of specific promoter subregions such as 5'UTR, exon 1 and intron 1 (+7 to +622), transcription factor binding sites and promoter elements like NF1HCS. Furthermore, we detected significant intra-pair differences in cytosine methylation for the region from -249 to -234 with regard to discordance for optic glioma with a higher grade of methylation in glioma cases. In conclusion, our findings of epigenetic differences of the NF1 promoter in leukocytes within mono zygotic twin pairs may serve as a proof of principle for other tissues. The results point towards a role of methylation patterns of the normal NF1 allele for expression differences and for modification of the NF1 phenotype.

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Language(s): eng - English
 Dates: 2010-12-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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Title: Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies.
  Alternative Title : Twin Res Hum Genet
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 13 (6) Sequence Number: - Start / End Page: 582 - 594 Identifier: ISSN: 1832-4274