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  Proteomic and functional analysis of the mitotic Drosophila centrosome.

Müller, H., Schmidt, D., Steinbrink, S., Mirgorodskaya, E., Lehmann, V., Habermann, K., et al. (2010). Proteomic and functional analysis of the mitotic Drosophila centrosome. The EMBO Journal, 29(19), 3344-3357. doi:10.1038/emboj.2010.210.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7A2C-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7A2D-D
Genre: Journal Article
Alternative Title : EMBO J

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 Creators:
Müller, H.1, Author              
Schmidt, D., Author
Steinbrink, S., Author
Mirgorodskaya, E.2, Author
Lehmann, V.2, Author
Habermann, K.1, Author              
Dreher, F.3, Author              
Gustavsson, N.2, Author
Kessler, T.4, Author              
Lehrach, H.1, Author              
Herwig, R.2, Author
Gobom, J.1, Author              
Ploubidou, A.2, Author
Boutros, M., Author
Lange, B. M.2, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Max Planck Society, ou_persistent13              
3Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              
4Systems Biology (Christoph Wierling), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479656              

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Free keywords: cell cycle; centrosome; Drosophila; mitosis; proteomics
 Abstract: Regulation of centrosome structure, duplication and segregation is integrated into cellular pathways that control cell cycle progression and growth. As part of these pathways, numerous proteins with well-established non-centrosomal localization and function associate with the centrosome to fulfill regulatory functions. In turn, classical centrosomal components take up functional and structural roles as part of other cellular organelles and compartments. Thus, although a comprehensive inventory of centrosome components is missing, emerging evidence indicates that its molecular composition reflects the complexity of its functions. We analysed the Drosophila embryonic centrosomal proteome using immunoisolation in combination with mass spectrometry. The 251 identified components were functionally characterized by RNA interference. Among those, a core group of 11 proteins was critical for centrosome structure maintenance. Depletion of any of these proteins in Drosophila SL2 cells resulted in centrosome disintegration, revealing a molecular dependency of centrosome structure on components of the protein translation machinery, actin- and RNA-binding proteins. In total, we assigned novel centrosome-related functions to 24 proteins and confirmed 13 of these in human cells.

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Language(s): eng - English
 Dates: 2010-10-06
 Publication Status: Published in print
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Title: The EMBO Journal
  Alternative Title : EMBO J
Source Genre: Journal
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Pages: - Volume / Issue: 29 (19) Sequence Number: - Start / End Page: 3344 - 3357 Identifier: ISSN: 0261-4189