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  High-throughput Universal Probe Salmonella Serotyping (UPSS) by nanoPCR.

Mertes, F., Biens, K., Lehrach, H., Wagner, M., & Dahl, A. (2010). High-throughput Universal Probe Salmonella Serotyping (UPSS) by nanoPCR. J Microbiol Methods, 83(2), 217-223. doi:10.1016/j.mimet.2010.09.005.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7A42-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7A43-A
Genre: Journal Article
Alternative Title : Journal of Microbiological Methods

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 Creators:
Mertes, F.1, Author              
Biens, K.2, Author
Lehrach, H.1, Author              
Wagner, M., Author
Dahl, A.1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Max Planck Society, ou_persistent13              

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Free keywords: Salmonella serotyping; Nano liter PCR; TaqMan; Universal reporter; High-throughput; Routine testing
 Abstract: Salmonella enterica subsp. enterica serovar identification is of great importance with respect to outbreak monitoring and case verification. Therefore rapid, sensitive and cost efficient detection of Salmonella spp. is indispensable within microbiology labs. To amalgamate single tube isolate identification with Salmonella typing, we developed the high-throughput Universal Probe Salmonella Serotyping (UPSS) technique based on nano liter PCR. In comparison to the classical approach, where O- and H-antisera are applied, the UPSS relies on specific gene content amplification of Salmonella spp. by a universal TaqMan assay for all markers and identification of the specific amplicon pattern. To enable high-throughput technology we employed a chip format containing 1024 wells loaded by an automated liquid-handling system which allowed us to perform TaqMan PCR reactions in volumes of 100nL per well. Herein we present proof of principle of the UPSS method by the use of a test panel of 100 previously serotyped Salmonella isolates to successfully verify the usability, accuracy and feasibility of the newly developed UPSS approach. We found that the methodology of the UPSS technology is capable of unequivocally identifying 30 Salmonella serotypes on a single chip within 3 hours but can be highly parallelized by the use of multiple PCR machines. Therefore the UPSS method offers a robust and straightforward molecular alternative for Salmonella detection and typing that saves expensive chemistry and can be easily automated.

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Language(s): eng - English
 Dates: 2010-09-24
 Publication Status: Published in print
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Title: J Microbiol Methods
  Alternative Title : Journal of Microbiological Methods
Source Genre: Journal
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Pages: - Volume / Issue: 83 (2) Sequence Number: - Start / End Page: 217 - 223 Identifier: ISSN: 0167-7012