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  Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae

Kube, M., Migdoll, A. M., Gehring, I., Heitmann, K., Mayer, Y., Kuhl, H., et al. (2010). Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae. BMC Genomics, 11, 11:393-11:393. doi:10.1186/1471-2164-11-393.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7ACE-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7ACF-0
Genre: Journal Article

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 Creators:
Kube, Michael1, Author              
Migdoll, Alexander M.2, Author
Gehring, Isabel, Author
Heitmann, Katja2, Author
Mayer, Yvonne2, Author
Kuhl, Heiner3, Author              
Knaust, Florian2, Author
Geider, Klaus, Author
Reinhardt, Richard1, Author              
Affiliations:
1High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              
2Max Planck Society, ou_persistent13              
3Sequencing (Head: Bernd Timmermann), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479670              

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 Abstract: Background The genus Erwinia includes plant-associated pathogenic and non-pathogenic Enterobacteria. Important pathogens such as Erwinia amylovora, the causative agent of fire blight and E. pyrifoliae causing bacterial shoot blight of pear in Asia belong to this genus. The species E. tasmaniensis and E. billingiae are epiphytic bacteria and may represent antagonists for biocontrol of fire blight. The presence of genes that are putatively involved in virulence in E. amylovora and E. pyrifoliae is of special interest for these species in consequence. Results Here we provide the complete genome sequences of the pathogenic E. pyrifoliae strain Ep1/96 with a size of 4.1 Mb and of the non-pathogenic species E. billingiae strain Eb661 with a size of 5.4 Mb, de novo determined by conventional Sanger sequencing and next generation sequencing techniques. Genome comparison reveals large inversions resulting from homologous recombination events. Furthermore, comparison of deduced proteins highlights a relation of E. billingiae strain Eb661 to E. tasmaniensis strain Et1/99 and a distance to E. pyrifoliae for the overall gene content as well as for the presence of encoded proteins representing virulence factors for the pathogenic species. Pathogenicity of E. pyrifoliae is supposed to have evolved by accumulation of potential virulence factors. E. pyrifoliae carries factors for type III secretion and cell invasion. Other genes described as virulence factors for E. amylovora are involved in the production of exopolysaccharides, the utilization of plant metabolites such as sorbitol and sucrose. Some virulence-associated genes of the pathogenic species are present in E. tasmaniensis but mostly absent in E. billingiae. Conclusion The data of the genome analyses correspond to the pathogenic lifestyle of E. pyrifoliae and underlines the epiphytic localization of E. tasmaniensis and E. billingiae as a saprophyte.

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Language(s): eng - English
 Dates: 2010-06-22
 Publication Status: Published in print
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Title: BMC Genomics
Source Genre: Journal
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Pages: - Volume / Issue: 11 Sequence Number: - Start / End Page: 11:393 - 11:393 Identifier: ISSN: 1471-2164