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  Proteomic analysis of sea urchin (Strongylocentrotus purpuratus) spicule matrix.

Mann, K., Wilt, F. H., & Poustka, A. J. (2010). Proteomic analysis of sea urchin (Strongylocentrotus purpuratus) spicule matrix. Proteome Science, 8, 33-45. doi:10.1186/1477-5956-8-33.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7AD1-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7AD2-6
Genre: Journal Article
Alternative Title : Proteome Sci

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Mann, K.1, Author
Wilt, F. H., Author
Poustka, A. J.2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Evolution and Development (Albert Poustka), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479650              

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 Abstract: ABSTRACT: BACKGROUND: The sea urchin embryo has been an important model organism in developmental biology for more than a century. This is due to its relatively simple construction, translucent appearance, and the possibility to follow the fate of individual cells as development to the pluteus larva proceeds. Because the larvae contain tiny calcitic skeletal elements, the spicules, they are also important model organisms for biomineralization research. Similar to other biominerals the spicule contains an organic matrix, which is thought to play an important role in its formation. However, only few spicule matrix proteins were identified previously. RESULTS: Using mass spectrometry-based methods we have identified 231 proteins in the matrix of the S. purpuratus spicule matrix. Approximately two thirds of the identified proteins are either known or predicted to be extracellular proteins or transmembrane proteins with large ectodomains. The ectodomains may have been solubilized by partial proteolysis and subsequently integrated into the growing spicule. The most abundant protein of the spicule matrix is SM50. SM50-related proteins, SM30-related proteins, MSP130 and related proteins, matrix metalloproteases and carbonic anhydrase are among the most abundant components. CONCLUSIONS: The spicule matrix is a relatively complex mixture of proteins not only containing matrix-specific proteins with a function in matrix assembly or mineralization, but also: 1) proteins possibly important for the formation of the continuous membrane delineating the mineralization space; 2) proteins for secretory processes delivering proteinaceous or non-proteinaceous precursors; 3) or proteins reflecting signaling events at the cell/matrix interface. Comparison of the proteomes of different skeletal matrices allows prediction of proteins of general importance for mineralization in sea urchins, such as SM50, SM30-E, SM29 or MSP130. The comparisons also help point out putative tissue-specific proteins, such as tooth phosphodontin or specific spicule matrix metalloproteases of the MMP18/19 group. Furthermore, the direct sequence analysis of peptides by MS/MS validates many predicted genes and confirms the existence of the corresponding proteins.

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Language(s): eng - English
 Dates: 2010-06-17
 Publication Status: Published in print
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Title: Proteome Science
  Alternative Title : Proteome Sci
Source Genre: Journal
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Pages: - Volume / Issue: 8 Sequence Number: - Start / End Page: 33 - 45 Identifier: ISSN: 1477-5956