English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  TRPV1 acts as a synaptic protein and regulates vesicle recycling.

Goswami, C., Rademacher, N., Smalla, K.-H., Kalscheuer, V. M., Ropers, H.-H., Gundelfinger, E. D., et al. (2010). TRPV1 acts as a synaptic protein and regulates vesicle recycling. Journal of Cell Science, 123(12), 2045-2057. doi:10.1242/jcs.065144.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7B0F-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7B10-3
Genre: Journal Article
Alternative Title : J. Cell. Sci.

Files

show Files
hide Files
:
2045.pdf (Any fulltext), 5MB
Name:
2045.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: PUBLIC
License:
-

Locators

show

Creators

show
hide
 Creators:
Goswami, Chandan1, Author              
Rademacher, Nils1, Author              
Smalla, Karl-Heinz, Author
Kalscheuer, Vera M.2, Author              
Ropers, Hans-Hilger1, Author              
Gundelfinger, Eckart D., Author
Hucho, Tim3, Author              
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              
2Chromosome Rearrangements and Disease (Vera Kalscheuer), Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479642              
3Signal Transduction in Mental Retardation and Pain (Tim Hucho), Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479646              

Content

show
hide
Free keywords: Capsaicin receptor; Synapse; Active zone; FM4-64 dye; Synaptic-vesicle recycling
 Abstract: Electrophysiological studies demonstrate that transient receptor potential vanilloid subtype 1 (TRPV1) is involved in neuronal transmission. Although it is expressed in the peripheral as well as the central nervous system, the questions remain whether TRPV1 is present in synaptic structures and whether it is involved in synaptic processes. In the present study we gathered evidence that TRPV1 can be detected in spines of cortical neurons, that it colocalizes with both pre- and postsynaptic proteins, and that it regulates spine morphology. Moreover, TRPV1 is also present in biochemically prepared synaptosomes endogenously. In F11 cells, a cell line derived from dorsal-root-ganglion neurons, TRPV1 is enriched in the tips of elongated filopodia and also at sites of cell-cell contact. In addition, we also detected TRPV1 in synaptic transport vesicles, and in transport packets within filopodia and neurites. Using FM4-64 dye, we demonstrate that recycling and/or fusion of these vesicles can be rapidly modulated by TRPV1 activation, leading to rapid reorganization of filopodial structure. These data suggest that TRPV1 is involved in processes such as neuronal network formation, synapse modulation and release of synaptic transmitters.

Details

show
hide
Language(s): eng - English
 Dates: 2010-05-18
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Cell Science
  Alternative Title : J. Cell. Sci.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 123 (12) Sequence Number: - Start / End Page: 2045 - 2057 Identifier: ISSN: 0021-9533