English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-throughput identification of genetic markers using representational oligonucleotide microarray analysis.

Lange, C., Mittermayr, L., Dohm, J. C., Holtgräwe, D., Weisshaar, B., & Himmelbauer, H. (2010). High-throughput identification of genetic markers using representational oligonucleotide microarray analysis. Theoretical and Applied Genetics, 121(3), 549-565. doi:10.1007/s00122-010-1329-2.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7B62-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7B63-7
Genre: Journal Article
Alternative Title : Theor Appl Genet

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Lange, C.1, Author              
Mittermayr, L.2, Author
Dohm, J. C.1, Author              
Holtgräwe, D., Author
Weisshaar, B., Author
Himmelbauer, H.1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Max Planck Society, ou_persistent13              

Content

show
hide
Free keywords: Beta vulgaris/*genetics/growth & development; Biological Markers/*metabolism; Chromosome Mapping; Gene Expression Profiling; Genetic Markers/*genetics; Linkage (Genetics); Molecular Sequence Data; Oligonucleotide Array Sequence Analysis
 Abstract: We describe a novel approach for high-throughput development of genetic markers using representational oligonucleotide microarray analysis. We test the performance of the method in sugar beet (Beta vulgaris L.) as a model for crop plants with little sequence information available. Genomic representations of both parents of a mapping population were hybridized on microarrays containing in total 146,554 custom made oligonucleotides based on sugar beet bacterial artificial chromosome (BAC) end sequences and expressed sequence tags (ESTs). Oligonucleotides showing a signal with one parental line only, were selected as potential marker candidates and placed onto an array, designed for genotyping of 184 F(2) individuals from the mapping population. Utilizing known co-dominant anchor markers we obtained 511 new dominant markers (392 derived from BAC end sequences, and 119 from ESTs) distributed over all nine sugar beet linkage groups and calculated genetic maps. Further improvements for large-scale application of the approach are discussed and its feasibility for the cost-effective and flexible generation of genetic markers is presented.

Details

show
hide
Language(s): eng - English
 Dates: 2010-04-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Theoretical and Applied Genetics
  Alternative Title : Theor Appl Genet
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 121 (3) Sequence Number: - Start / End Page: 549 - 565 Identifier: ISSN: 0040-5752