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  A transcriptional roadmap to the induction of pluripotency in somatic cells

Wang, Y., Mah, N., Prigione, A., Wolfrum, K., Andrade-Navarro, M. A., & Adjaye, J. (2010). A transcriptional roadmap to the induction of pluripotency in somatic cells. doi:10.1007/s12015-010-9137-2.

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 Creators:
Wang, Y.1, Author           
Mah, N., Author
Prigione, A.1, Author           
Wolfrum, K.1, Author           
Andrade-Navarro, M. A., Author
Adjaye, J.1, Author           
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              

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Free keywords: Cellular reprogramming EMT MET Gene regulatory networks iPS cells ES cells PiPSC Self-renewal Pluripotency
 Abstract: Human embryonic stem (ES) cells possess an enormous potential for applications in regenerative medicine. However, these cells have several inevitable hurdles limiting their clinical applications, such as transplant rejection and embryo destruction. A milestone recently achieved was the derivation of induced pluripotent stem (iPS) cells by over-expressing combinations of defined transcription factors, namely, OCT4, SOX2, NANOG, and LIN28 or OCT4, SOX2, KLF4, and c-MYC. Human iPS cells exhibit many characteristics identical to those of inner cell mass-derived ES cells. Here, we summarize the generation of human fibroblast-derived iPS cells and discuss the promises and limitations of their use. In addition, by utilising numerous published transcriptome datasets related to ES cells, fibroblast-derived iPS cells, partially induced pluripotent stem cells (PiPSC) and wild type fibroblasts, we reveal similarities (self-renewal signature) and differences (donor cell-type and PiPSC signatures) in genes and associated signaling pathways operative in the induction of pluripotency in fibroblasts. In particular, we highlight that induction of ground state pluripotency is also favoured by the inhibition of epithelial mesenchymal transition (EMT) and hence the induction of mesenchymal epithelial transition (MET). We anticipate that these findings might aid in the establishment of more efficient protocols for inducing pluripotency in somatic cells.

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Language(s): eng - English
 Dates: 2010-03-24
 Publication Status: Published in print
 Pages: -
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